Fecha/hora: 20/05/2024 14:42:22

Biblioteca(s): 	INIA Las Brujas.	
Fecha corriente: 	23/07/2021	
Fecha de la última actualización: 	23/07/2021	
Autoria: 	OLIVARO, C.; ESCOBAL, M.; DE SOUZA, G.; MEDEROS, A.	
Afiliación: 	CRISTINA OLIVARO, Espacio de Ciencia y Tecnología Química, Centro Universitario Regional Noreste, Universidad de la República, Tacuarembó, Uruguay; MICAELA ESCOBAL, Espacio de Ciencia y Tecnología Química, Centro Universitario Regional Noreste, Universidad de la República, Tacuarembó, Uruguay; GUILLERMO DE SOUZA, Espacio de Ciencia y Tecnología Química, Centro Universitario Regional Noreste, Universidad de la República, Tacuarembó, Uruguay; AMERICA ESTHER MEDEROS SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.	
Título: 	Chemical characterisation and in vitro anthelmintic activity of phenolic-rich extracts from the leaves and branches of Maytenus ilicifolia, a native plant from South America.	
Complemento del título: 	Short Communication.	
Año de publicación: 	2021	
Fuente/Imprenta: 	Natural Product Research, 2021. Doi: https://doi.org/10.1080/14786419.2021.1948844	
DOI: 	10.1080/14786419.2021.1948844	
Idioma: 	Inglés	
Notas: 	Article history: Received 28 Mar 2021, Accepted 20 Jun 2021, Published online: 06 Jul 2021.
Correspondence: Cristina Olivaro, emai: cristina.olivaro@cut.edu.uy   -     
Supplemental data for this article can be accessed online at https://www.tandfonline.com/doi/suppl/10.1080/14786419.2021.1948844
This work was supported by the INIA under Grant INIA CL_40 and PEDECIBA. Instituto Nacional de Investigación Agropecuaria (INIA); Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).	
Contenido: 	ABSTRACT - Free (epi) catechin, quercetin, (epi) gallocatechin, flavonol glycosides and condensed tannins were identified according to their molecular mass, characteristic product ions and retention times in extracts obtained from leaves and branches of Maytenus ilicifolia (Congorosa) by mass spectrometry. The in vitro anthelmintic activity against cattle gastrointestinal nematodes of Congorosa extract was determined using the Egg Hatch Inhibition Assay. Additionally, commercial quercetin, gallocatechin and epicatechin were evaluated. Although total phenolics, total tannins and condensed tannins contents were lower in branches extract than in leaves extract, the EC50 were 0.065mg/mL and 0.890 mg/mL for branches and leaves extract, respectively. Moreover, the use of polyvinylpyrrolidone as a blocking agent of tannins, did not change significantly the EC50 for branches extract, but significantly changed for leaves extract. Quercetin and gallocatechin EC50 values were in the range 0.03–0.05 mg/mL and epicatechin showed 100% inhibition of the egg hatching process at 0.004mg/mL.
Copyright © 2021 Informa UK Limited, trading as Taylor & Francis Group	
Palabras-Clave: 	Egg hatch inhibition assay; Mass spectrometry; Maytenus ilicifolia; Phenolic compounds; Ruminants anthelmintic activity.	
Categoría del tema: 	L73 Enfermedades de los animales	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (102801-1) - PXI - AP - DD - PP/Natural Product Research/2021 -  -  - DIS